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In the first experiment, a varied concentration of primary antibody GAR-IgG was immobilized on to reduced fibers activated by glutaraldehyde. Analyte RAGIgG was immobilized with a fixed conc. of 60 ng/mL and then serially diluted secondary Ab conjugate GAR-HRP was incubated with the fibers. The assay was carried out in triplicate and the averages of the results are presented in Fig 10 and 11. 10) was observed. D. for 3 µg/mLGAR-IgG conc. at 1:8000 conjugate dilutions, thus, these values were established as the optimized concentration and dilution.

1997). New Directions in Diagnosis. 16, No. 3, (March 1997), pp. S43-S48 Gosling, J. P. (1990). A decade of development in immunoassay methodology. Clinical Chemistry, Vol. 36, No. , Bajaj, P. and Sen, K. (2003). Thermal behavior of acrylonitrile carboxylic acid copolymers. 88, No. 3, (18, April), pp. , Mulder, M. and Smolders, C. (1994). Surface amination of poly(acrylonitrile). Advances in Colloid and Interface Science, Vol. 52, (19 September 1994), pp. , and Okada, K. I. (1995). A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzymelinked immunosorbent assay.

1989]. Fig. 1. ATR-FTIR spectra of unmodified and aminated PAN fibers Fig. 2. 3 Differential scanning calorimetry Thermal properties of unmodified and reduced PAN fibers were studied in DSC and are given in Fig. 3, 4 and Table 2. It was observed that two thermal transitions occurred for dry PAN fibers in the first cycle of heating and only one in the second cycle. On first cycle of heating a transition is observed at 96 0C. A sharp peak also appears at 150 0C which is attributed to the cyclization of PAN (Fig.

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